How can I get sharper close-up photos of ice crystals with a 60mm macro lens?

Asked 11/13/2019

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I'm photographing ice crystals on a flat surface in a lab using a Nikon D3400 with an AF Micro-Nikkor 60mm f/2.8D and controlled LED lighting. The camera is mounted above the subject, moved by a stepper motor, and it stops completely before each exposure. I need very fine detail and more depth of field, but my images are not sharp enough.

I’ve been trying settings around 1/30 s and f/22, assuming the slow shutter speed is acceptable because the subject and camera are stationary. I also want to understand the lens’s minimum focus distance specification (0.72 ft): does that refer to the closest focusing distance from the camera/sensor to the subject?

What is the best way to focus for this kind of macro work, and what settings or techniques will improve sharpness and usable depth of field?

Originally by Photography Stack Exchange contributor. Source · Licensed CC BY-SA 4.0

Photography Stack Exchange contributor

6y ago

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The minimum focus distance is indeed the minimum distance you'll need to take (from sensor to subject) to be able to focus. The lens can't focus closer than that. A way to get around this is with an extension tube which lets you move the lens closer to the subject and increases magnification at the cost of some light.

If the ice is stable and you have the camera firmly mounted you can go with slow shutter speeds, possibly slower than 1/30. If that helps to use a narrower aperture (higher f-stop) then you could increase the depth of field that way. You can also try to reduce vibrations by using a remote shutter button and the mirror lockup function if the camera has it.

Even with all that, macro shots can have very, very thin depths of field. A flat-looking ice crystal might still have too much relief to get everything as sharp as you want. At that point it's interesting to look into the technique of focus stacking. This means taking multiple pictures at different focus distances (although still very tiny differences in absolute terms) and then having parts of those pictures combined to get one image with substantially more in focus. Your subject has the advantage of not suddenly flying away or moving around like with plants or animals, which can make the technique easier to apply.

Originally by user78344. Source · Licensed CC BY-SA 4.0

user78344

6y ago

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Yes—minimum focus distance is the closest distance at which the lens can focus, measured to the sensor plane, not the front of the lens.

If the ice and camera are truly stationary, slow shutter speeds are fine, even slower than 1/30 s, as long as vibration is controlled. Use a firm mount, remote release, and mirror lock-up if available.

The bigger issue is likely aperture: at macro distances, depth of field is always very thin, and f/22 often reduces sharpness because of diffraction. For better detail, try working around f/5.6 to f/8 and focus manually using magnified Live View.

If you need to focus even closer or increase magnification, an extension tube can help, though it reduces the light reaching the sensor.

So the practical approach is: use a stable setup, manual focus in Live View, a moderate aperture instead of f/22, and compensate with longer exposure if needed. That usually gives sharper macro results than stopping down all the way.

UniqueBot

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6y ago

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