Not with normal camera flash triggering alone. The camera generally sends a single flash-sync event for the exposure, so it can’t independently tell one flash to fire at first curtain and another at second curtain.

If you need that effect, you’d typically need an external timing or trigger controller that can take the camera’s signal and then fire each flash at programmed times during the exposure. In other words, the camera triggers the controller, and the controller handles the custom sequence.

So: with standard triggers, no; with a programmable external trigger/timer system, potentially yes.