What equipment is needed for photomicrography at around 9x magnification?
Asked 10/19/2020
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I’ve seen images such as the 9x magnification photo of nylon stockings on Nikon Small World and would like to understand what equipment is typically required to make similar photos or videos. I’m especially interested in whether this can be done with a camera-based setup rather than a full microscope system. If polarized-light effects are part of the look, what extra equipment is needed? I’d also like to know whether there are lower-cost hobby approaches.
Originally by Photography Stack Exchange contributor. Source · Licensed CC BY-SA 4.0
Photography Stack Exchange contributor
5y ago
2 Answers
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Details are described in the Nikon Microscopy University site.
Essentially, you need a microscope that includes a special lightsource designed for polarized light observations, a polarizer, special lenses with interference patterns, rotating stage to get the correct angle, and a digital camera system and extension tube.
It might not surprise you that Nikon is a significant vendor and manufacturer of microscopy solutions.
I doubt this is in many photographer's 'hobby budget'. Prices of these are not published, but used equipment on eBay are priced at $18,000+ USD.
Originally by user4880. Source · Licensed CC BY-SA 4.0
user4880
5y ago
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Generated from our catalog & community — verify before relying on it.
For images like that Nikon Small World example, the usual setup is a microscope-based system rather than a standard camera-and-lens macro setup. The cited example typically involves polarized-light microscopy, which adds specialized gear: a suitable microscope, a polarized light source, polarizer/analyzer elements, optics that produce interference colors, a rotating stage to control angle, and a camera attached via an adapter or extension tube.
If you want a lower-cost hobby route, you can experiment. Around 9x magnification is high but not impossible with improvised setups: an educational microscope plus a camera attachment can work, or a camera with a bellows between body and lens to gain more magnification than normal macro accessories. Bellows magnification depends on lens focal length and extension.
Polarized-light effects are optional, but if you want that look, you can try simple polarized light sources and filters as a DIY starting point. Results won’t match a dedicated microscopy rig, but it can be a good way to learn before investing in specialized equipment.
In short: for true photomicrography like the example, start with a microscope system; for experimentation, try a basic microscope or a bellows-based camera setup.
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