How can I photograph 0.05–0.1 mm particles embedded inside 6 mm-thick glass?
Asked 9/1/2013
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I need to photograph very small particles embedded in glass, about 0.05–0.1 mm in diameter. The sample is roughly 6 mm thick and cannot be cut. I need enough resolution to see the surface character of the particle. What type of microscope and camera setup would work best, and are there any lighting tips for this kind of subject?
Originally by Photography Stack Exchange contributor. Source · Licensed CC BY-SA 4.0
Photography Stack Exchange contributor
12y ago
2 Answers
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You can buy adapters for just about any DSLR mount that will allow you to resolve a microscopic image onto a camera sensor. They are pretty easy to find on just about any microscope store's website. Expect to spend anywhere from $40 to $400 depending on the quality you want. They most commonly go in place of the eyepiece of the microscope.
Originally by user11392. Source · Licensed CC BY-SA 4.0
user11392
12y ago
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A practical approach is a low-magnification dissecting (stereo) microscope rather than a high-power compound microscope. For particles in the 0.05–0.1 mm range, a dissecting microscope can give you useful magnification while providing much more working distance and depth of field, which matters when the particle is embedded inside 6 mm-thick glass.
For imaging, you can attach a DSLR or similar camera to the microscope using a microscope-to-camera adapter; these commonly replace the eyepiece or connect through the microscope’s photo port.
Lighting is critical. Instead of only front-lighting the sample, illuminate the edges of the glass so the glass acts like a light pipe and carries light to the embedded particle. Lighting from multiple edges can help reveal surface texture and improve contrast. Keep the background dark to increase visibility, especially for light or translucent particles.
So the key setup is: stereo/dissecting microscope + camera adapter + careful edge lighting of the glass.
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