Does a microscope’s top-mounted digital camera use eyepiece magnification?
Asked 10/23/2014
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On microscopes that have a camera mounted on the top trinocular port, does the camera image include the eyepiece magnification or bypass it? For example, if the microscope has a 40x objective and a 10x eyepiece, the visual magnification would be 400x. Would the digital image show the same magnification? Or is it better to mount a camera through an eyepiece instead?
Originally by kenorb. Source · Licensed CC BY-SA 4.0
kenorb
11y ago
2 Answers
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But does it include or bypass eyepiece magnification?
As the camera in the included image is clearly not looking through either of the eyepiece, it obviously doesn't include magnification due to those eyepieces. The camera likely has its own lens, however, that may provide a similar overall effect. For example, it looks like most of the AmScope cameras include a 0.5x reduction lens, which is probably necessary to get the image from the objective to fit on the (relatively small) image sensor.
If not, it's better to attach camera directly into eyepiece then?
When you use a camera with a microscope like this, the camera generally takes the place of the eyepiece. That is, you remove the eyepiece first, and the camera fits into 23.2mm or 30mm ID tube where the eyepiece was. You should get about the same results this way as you would using the camera port.
Originally by user4262. Source · Licensed CC BY-SA 4.0
user4262
11y ago
0
Generated from our catalog & community — verify before relying on it.
A top-mounted microscope camera usually does not use the eyepiece magnification unless it is literally imaging through an eyepiece. On a trinocular microscope, the camera typically connects to a separate photo tube and effectively replaces the eyepiece in that path.
So the camera image is not simply “40x objective × 10x eyepiece = 400x.” Instead, the final field of view depends on the objective, the microscope’s optical path, the camera sensor size, and any relay/reduction lens fitted to the camera adapter. Many microscope cameras use a reduction lens such as 0.5x so the image fits the small sensor.
Attaching a camera through an eyepiece is possible, but it is generally not the preferred approach if the microscope already has a proper camera port. A dedicated camera tube is usually the cleaner and more standard setup.
Also, for digital imaging, “magnification” is less useful than field of view and captured detail, because the apparent size also depends on the screen you view the image on.
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